Mishra N K, Wilson C M, Pant K J, Thomas F O
J Toxicol Environ Health. 1978 Jan;4(1):79-91. doi: 10.1080/15287397809529646.
Thirteen chemicals, eight carcinogenic and five closely related noncarcinogenic analogues, were tested to establish the validity of a simultaneous procedure for the in vitro assay of potential carcinogens and mutagens. The assay utilizes Fischer rat embryo (FRE) cells infected with Rauscher leukemia virus and simultaneously measures the induction of cellular transformation (growth in soft agar) and mutagenesis (ouabain resistance) by chemicals. An activation procedure for the metabolic conversion of the procarcinogens and promutagens to biologically active forms is described. All chemicals that produced transformation in the FRE system also induced a significant increase in ouabain resistance. The significance and the potential uses of this assay are discussed.
对13种化学物质（8种致癌物质和5种密切相关的非致癌类似物）进行了测试，以确定一种同时检测潜在致癌物和诱变剂的体外试验方法的有效性。该试验利用感染劳氏肉瘤病毒的费氏大鼠胚胎（FRE）细胞，同时检测化学物质对细胞转化（软琼脂中生长）和诱变（哇巴因抗性）的诱导作用。描述了一种将前致癌物和前诱变剂代谢转化为生物活性形式的激活程序。在FRE系统中产生转化的所有化学物质也都显著增加了哇巴因抗性。讨论了该试验的意义和潜在用途。
