Dunkel V C, Pienta R J, Sivak A, Traul K A
J Natl Cancer Inst. 1981 Dec;67(6):1303-12.
This study provides a preliminary comparative evaluation of the responses to a series of 49 chemicals, in in vitro transformation assays, of Balb/3T3 cells, Syrian hamster embryo cells, and Fischer 344 rat embryo cells infected with Rauscher murine leukemia virus. The chemicals assayed included aromatic amines; polycyclic aromatic hydrocarbons; alkylating agents; nitrosamines, hydrazines, and related compounds; heterocyclic compounds; amides, ureas, and acylating agents; inorganic compounds; and hormones. In all three assays 37 of the chemicals were tested. The most uniform test responses were obtained with the polycyclic aromatic hydrocarbons and inorganic compounds With the other groups of chemicals, more variation in response was observed. This study expands the base of information on the potential of these in vitro transformation systems, and the lack of responses with some of the chemicals underscores the need for incorporation of exogenous metabolic activating systems into these assay systems.
本研究对感染劳斯氏鼠白血病病毒的Balb/3T3细胞、叙利亚仓鼠胚胎细胞和Fischer 344大鼠胚胎细胞在体外转化试验中对一系列49种化学物质的反应进行了初步比较评估。所检测的化学物质包括芳香胺；多环芳烃；烷化剂；亚硝胺、肼及相关化合物；杂环化合物；酰胺、脲和酰化剂；无机化合物；以及激素。在所有这三种试验中，对37种化学物质进行了测试。多环芳烃和无机化合物的测试反应最为一致。对于其他化学物质组，观察到的反应变化更大。本研究扩展了关于这些体外转化系统潜力的信息基础，一些化学物质缺乏反应突出了在这些检测系统中加入外源性代谢激活系统的必要性。
