Sazykin A Iu, Navashin S M
Antibiotiki. 1984 Sep;29(9):657-61.
The substrate profiles of beta-lactamases defected in 46 clinical polyresistant strains of gram-negative bacteria were determined. By the substrate profile and sensitivity to inhibitors (dicloxacillin and p-CMB) beta-lactamases were considered to belong to classes I, II, III, IV and V of the Richmond classification. The molecular weights of the enzymes were measured. Enterobacter aerogenes 6803, Enterobacter aerogenes 11030 and Klebsiella pneumoniae 970 produced simultaneously two beta-lactamases belonging to different classes. beta-Lactamases of classes I and III were detected in the cells of Enterobacter aerogenes 6803. The cells of Enterobacter aerogenes 11030 contained beta-lactamases of classes V and III and the cells of Klebsiella pneumonia 970 beta-lactamases of classes II and III. Therefore, in all the cases one of beta-lactamases belonged to the class III enzyme close to TEM beta-lactamases by its substrate profile, molecular weight and sensitivity to the inhibitors. Cephalexin and dicloxacillin were most frequently stable to the effect of the above beta-lactamases. The enzymes from 26 strains did not hydrolyse or hydrolysed slightly cephalexin and the enzymes from 19 strains did not hydrolyse of hydrolysed slightly dicloxacillin.
测定了46株革兰氏阴性临床多重耐药菌株中β-内酰胺酶的底物谱。根据底物谱和对抑制剂（双氯西林和对氯汞苯甲酸）的敏感性，β-内酰胺酶被认为属于里士满分类的I、II、III、IV和V类。测量了这些酶的分子量。产气肠杆菌6803、产气肠杆菌11030和肺炎克雷伯菌970同时产生两种属于不同类别的β-内酰胺酶。在产气肠杆菌6803细胞中检测到I类和III类β-内酰胺酶。产气肠杆菌11030细胞含有V类和III类β-内酰胺酶，肺炎克雷伯菌970细胞含有II类和III类β-内酰胺酶。因此，在所有情况下，其中一种β-内酰胺酶属于III类酶，其底物谱、分子量和对抑制剂的敏感性与TEMβ-内酰胺酶相近。头孢氨苄和双氯西林对上述β-内酰胺酶的作用最常表现为稳定。26株菌株的酶不水解或轻微水解头孢氨苄，19株菌株的酶不水解或轻微水解双氯西林。
