Kuppers R C, Simon M M, Eichmann K
Immunobiology. 1984 Oct;167(4):365-75. doi: 10.1016/S0171-2985(84)80008-X.
The growth properties of Con A activated, Lyt selected splenic T lymphocytes were examined by limiting dilution analysis and clonally by single cell picking. Under the conditions used, a comparable frequency of Lyt 1+ and Lyt 2+ cells grew after Con A activation in the presence of Con A rat spleen supernatant. At a clonal level, however, the growth of these subsets differed qualitatively and quantitatively. While Lyt 2+ cells obtained clone sizes of several hundred cells, Lyt 1+ clone sizes were usually less than 100 cells, and many clones aborted their growth after a few days. Morphologically, the Lyt 1+ cell was smaller and usually showed fewer pseudopodial protusions as compared to the Lyt 2+ cell.
通过有限稀释分析并通过单细胞挑选进行克隆，研究了伴刀豆球蛋白A（Con A）激活、Lyt分型的脾T淋巴细胞的生长特性。在所使用的条件下，在Con A大鼠脾上清液存在的情况下，Con A激活后，Lyt 1 +和Lyt 2 +细胞的生长频率相当。然而，在克隆水平上，这些亚群的生长在质量和数量上有所不同。虽然Lyt 2 +细胞获得了数百个细胞的克隆大小，但Lyt 1 +克隆大小通常小于100个细胞，并且许多克隆在几天后停止生长。形态学上，与Lyt 2 +细胞相比，Lyt 1 +细胞更小，通常显示出较少的伪足突起。
