Gullberg M, Larsson E L
J Immunol. 1983 Jul;131(1):19-22.
We investigated whether concanavalin A-stimulated, TCGF-reactive, and TCGF-producing T cells could be separated with regard to their Lyt phenotypes. Normal spleen cells, pretreated with monoclonal anti-Lyt-1 or anti-Lyt-2 antibodies and complement were assayed for their capacity to respond to TCGF, as well as for their ability to produce TCGF after Con A stimulation. The results demonstrate that reactivity to TCGF is predominantly induced in Lyt-2+ lymphocytes, because it is reduced by more than 95% in Lyt-1+23- T cell populations as compared with cultures containing Lyt-1-23+ cells. Limiting dilution analysis revealed that up to 20% of the cells in Lyt-2-containing populations were induced by Con A to TCGF-reactivity, whereas 20-fold fewer clones were induced in the Lyt-2-depleted cell populations. On the other hand, the study of TCGF production in Lyt-1-23+ populations revealed that the former population was considerably enriched in TCGF-producing T cells, whereas the latter was substantially depleted. We conclude that TCGF-producing and TCGF-reactive T cells are largely distinct lymphocyte populations that can be separated on the basis of their Lyt phenotypes.
我们研究了刀豆蛋白A刺激的、对TCGF有反应的以及产生TCGF的T细胞是否可以根据其Lyt表型进行分离。用单克隆抗Lyt-1或抗Lyt-2抗体及补体预处理的正常脾细胞，检测其对TCGF的反应能力以及在刀豆蛋白A刺激后产生TCGF的能力。结果表明，对TCGF的反应性主要在Lyt-2⁺淋巴细胞中诱导产生，因为与含有Lyt-1⁻23⁺细胞的培养物相比，Lyt-1⁺23⁻T细胞群体中的反应性降低了95%以上。有限稀释分析显示，含有Lyt-2的群体中高达20%的细胞被刀豆蛋白A诱导产生对TCGF的反应性，而在Lyt-2缺失的细胞群体中诱导产生的克隆数量减少了20倍。另一方面，对Lyt-1⁻23⁺群体中TCGF产生的研究表明，前一群体中产生TCGF的T细胞显著富集，而后者则大量减少。我们得出结论，产生TCGF的T细胞和对TCGF有反应的T细胞在很大程度上是不同的淋巴细胞群体，可以根据它们的Lyt表型进行分离。
