Scholer A, Hohenwallner W
J Clin Chem Clin Biochem. 1984 Oct;22(10):677-84. doi: 10.1515/cclm.1984.22.10.677.
A continuous method for the determination of alpha-amylase activity with a defined substrate has been published by Rauscher et al. in Berichte der Osterreichischen Gesellschaft für Klinische Chemie 4, 150 (1981). It offers the following advantages: good practicability, easy adaptation to analysers and short incubation time. Twenty-one laboratories took part in a multicentric study on this method, working with different instruments and manual procedures at three temperatures. Additionally, all laboratories participated in a laboratory improvement program, in order to discriminate systematic from accidental errors. Data on imprecision, linearity, interferences and comparability of the method have been presented at a workshop. The resulting coefficients of variation for imprecision in series as well as from day to day were 0.6-6.3% and 1.3-7.7%, respectively. No interferences from bilirubin, glucose, maltose and 37 selected drugs were found. The results from the described method correlate well with those from an UV-test (substrate: maltoheptaose); compared with other methods, however, the expected systematic deviations were observed. Reference values for 25, 30 and 37 degrees C are indicated in this report.
劳舍尔等人在《奥地利临床化学学会报告》第4卷，第150页（1981年）发表了一种用特定底物连续测定α淀粉酶活性的方法。该方法具有以下优点：实用性强、易于适配分析仪且孵育时间短。21个实验室参与了对该方法的多中心研究，在三个温度下使用不同仪器和手工操作程序。此外，所有实验室都参与了一项实验室改进计划，以区分系统误差和偶然误差。关于该方法的不精密度、线性、干扰和可比性的数据已在一次研讨会上公布。该方法批内和批间不精密度的变异系数分别为0.6% - 6.3%和1.3% - 7.7%。未发现胆红素、葡萄糖、麦芽糖和37种选定药物的干扰。所述方法的结果与紫外检测法（底物：麦芽七糖）的结果相关性良好；然而，与其他方法相比，观察到了预期的系统偏差。本报告给出了25℃、30℃和37℃时的参考值。
