Pike B L, Raubitschek A, Nossal G J
Proc Natl Acad Sci U S A. 1984 Dec;81(24):7917-21. doi: 10.1073/pnas.81.24.7917.
B lymphocytes specifically reactive to the hapten fluorescein (FLU) were prepared from normal adult murine spleen by the hapten-gelatin affinity procedure. They were placed in 10 microliter of microcultures singly or in small numbers in the absence of any feeder, filler, or accessory cell. The "T cell-independent" antigen FLU-conjugated polymerized flagellin (FLU-POL) or a selected batch of FLU-conjugated Ficoll were used, and these stimulated division and differentiation only in the concomitant presence of lymphokines acting as B-cell growth and differentiation factors (BGDF). It was found that human interleukin 2 (IL-2), prepared by recombinant DNA technology (r-IL-2), was an effective, albeit rather weak, BGDF in this system. When an IL-2-free source of BGDF was used with the antigenic stimulus, addition of r-IL-2 did not augment the response, nor did removal of IL-2 from the crude lymphokine mixture diminish the BGDF activity.
通过半抗原 - 明胶亲和程序从正常成年小鼠脾脏中制备出对半抗原荧光素（FLU）具有特异性反应的B淋巴细胞。将它们单独或以少量置于10微升的微量培养物中，且不存在任何饲养细胞、填充细胞或辅助细胞。使用了“非T细胞依赖性”抗原FLU偶联的聚合鞭毛蛋白（FLU - POL）或一批选定的FLU偶联的菲可立（Ficoll），并且这些抗原仅在作为B细胞生长和分化因子（BGDF）的淋巴因子同时存在的情况下才能刺激细胞分裂和分化。研究发现，通过重组DNA技术制备的人白细胞介素2（IL - 2）（r - IL - 2）在该系统中是一种有效的BGDF，尽管其作用相当微弱。当使用不含IL - 2的BGDF来源与抗原刺激物一起使用时，添加r - IL - 2并不会增强反应，从粗制淋巴因子混合物中去除IL - 2也不会降低BGDF活性。
