Leanderson T, Lundgren E, Ruuth E, Borg H, Persson H, Coutinho A
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7455-9. doi: 10.1073/pnas.79.23.7455.
A T-cell hybridoma was derived by somatic cell hybridization between concanavalin A-activated BALB/c spleen cells and the AKR thymoma BW 5147. Media conditioned by hybridoma cells, even at high dilutions (1:1,000) support the growth of lipopolysaccharide-stimulated B-cell blasts but not that of T-cell growth factor (TCGF)-reactive T-cells. This activity, herein designated B-cell growth factor (BCGF), has a Mr of approximately equal to 20,000 and it can readily be separated from TCGF (Mr approximately equal to 30,000) by gel filtration. BCGF is constitutively produced by the hybridoma cells, it is removed from conditioned media by incubation with target cells at +4 degrees C, and it is equally effective on B-cell blasts carrying different major histocompatibility complex and Ig haplotypes. BCGF shows no T-cell replacing factor (TRF) activity, and it is poor in supporting the development of Ig-secreting plaque-forming cells in B-cell blast cultures. Terminal maturation, however, can be induced in BCGF-dependent blasts by addition of conditioned media from normal helper T cell cultures, suggesting that two distinct factors are involved in the helper cell-dependent growth and maturation of B lymphocytes.
通过伴刀豆球蛋白A激活的BALB/c脾细胞与AKR胸腺瘤BW 5147之间的体细胞杂交，获得了一种T细胞杂交瘤。杂交瘤细胞条件培养基，即使在高稀释度（1:1000）下，也能支持脂多糖刺激的B细胞母细胞生长，但不能支持对T细胞生长因子（TCGF）有反应的T细胞生长。这种活性，在此称为B细胞生长因子（BCGF），其分子量约为20000，通过凝胶过滤可很容易地与TCGF（分子量约为30000）分离。BCGF由杂交瘤细胞组成性产生，通过在4℃下与靶细胞孵育可从条件培养基中去除，并且对携带不同主要组织相容性复合体和Ig单倍型的B细胞母细胞同样有效。BCGF不显示T细胞替代因子（TRF）活性，并且在支持B细胞母细胞培养物中分泌Ig的噬斑形成细胞的发育方面效果不佳。然而，通过添加来自正常辅助性T细胞培养物的条件培养基，可以在依赖BCGF的母细胞中诱导终末成熟，这表明两种不同的因子参与了辅助性细胞依赖的B淋巴细胞生长和成熟。
