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蛙卵母细胞的钾离子外流:温度、预孵育及注射明胶珠的影响

Potassium efflux from frog oocytes: effects of temperature, preincubation and injection of a gelatin bead.

作者信息

Edelmann L, Ochsenfeld M M, Negendank W

出版信息

Physiol Chem Phys Med NMR. 1984;16(3):197-207.

PMID:6334859
Abstract

42K efflux was studied in frog oocytes. 17 of 43 cells had only a single slow exponential exchange of 42K, while the remainder had a curvature of 42K exchange with one or more fast fractions preceding the slow exponential one. This variability in the characteristic of 42K efflux is not associated with evident damage, occurs at both 5 degrees and 25 degrees C., is independent of the method of preincubation, and occurs whether or not the cells are injected with a gelatin bead. Most of the fast-exchange fraction of K is not caused by follicle cells or other extraoocytic sources. These results may help explain some of the apparently conflicting results reported in studies of K exchange in amphibian oocytes.

摘要

在蛙卵母细胞中研究了42K外流情况。43个细胞中有17个细胞对42K仅有单一的缓慢指数性交换,而其余细胞的42K交换具有曲率,在缓慢指数性交换之前有一个或多个快速组分。42K外流特征的这种变异性与明显的损伤无关,在5摄氏度和25摄氏度时均会出现,与预孵育方法无关,且无论细胞是否注射了明胶珠都会出现。大部分K的快速交换组分并非由卵泡细胞或其他卵母细胞外来源引起。这些结果可能有助于解释两栖类卵母细胞K交换研究中报道的一些明显相互矛盾的结果。

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Physiol Chem Phys Med NMR. 1984;16(3):197-207.
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