Perrin D, Bignon J D, Beaujard E, Cheneau M L
Gastroenterol Clin Biol. 1984 Dec;8(12):907-10.
The characteristics, the origin and the role in the determinism and/or in the course of hepatocellular necrosis of immune disorders in patients with alcoholic liver cirrhosis still remain unclear. In this study, the T-lymphocyte population was determined by sheep-rosette formation and labelling with OKT3-monoclonal antibodies. T-cell subsets were investigated using OKT4 and OKT8 monoclonal antibodies in the peripheral blood of 40 alcoholic patients with cirrhosis (CA) and of 23 non-cirrhotic alcoholic (ANC) patients. The results were compared to those in 34 healthy volunteers. A decrease in the percentage of T-lymphocytes in the two groups of alcoholic subjects was noted. However, this decrease was significant only in the CA group and only when OKT3 antibodies were used as markers. The lymphocyte-subset identified by OKT4 antibodies was decreased as well in the CA and ANC groups; however the OKT8+ subset was significantly decreased in the CA group only (p less than 0.001). No correlation was found between this decrease in OKT8+ subpopulation or between the increased OKT4/OKT8 ratio and all biochemical parameters of hepatic function measured in this study. The significance of this T-cell imbalance in CA has still to be elucidated, concerning both the functional activity of the reduced pool of OKT8+ cells (suppressor or cytotoxic?) and the mechanism of this decrease.
酒精性肝硬化患者免疫紊乱在肝细胞坏死的决定因素和/或病程中的特征、起源及作用仍不清楚。在本研究中，通过绵羊红细胞花环形成试验及用OKT3单克隆抗体标记来测定T淋巴细胞群体。使用OKT4和OKT8单克隆抗体对40例酒精性肝硬化患者（CA）及23例非肝硬化酒精性患者（ANC）的外周血中的T细胞亚群进行了研究。并将结果与34名健康志愿者的结果进行比较。结果发现，两组酒精性受试者的T淋巴细胞百分比均有所下降。然而，仅在CA组中，且仅当使用OKT3抗体作为标志物时，这种下降才具有统计学意义。在CA组和ANC组中，由OKT4抗体识别的淋巴细胞亚群也有所减少；然而，仅在CA组中OKT8 +亚群显著减少（p小于0.001）。在本研究中，未发现OKT8 +亚群的这种减少或OKT4/OKT8比值增加与所有肝功能生化参数之间存在相关性。关于CA中这种T细胞失衡的意义，仍有待阐明，这涉及OKT8 +细胞减少群体的功能活性（抑制性还是细胞毒性？）以及这种减少的机制。
