Munabi A K, Cassorla F G, D'Agata R, Albertson B D, Loriaux D L, Lipsett M B
Steroids. 1984 Mar;43(3):325-31. doi: 10.1016/0039-128x(84)90050-3.
Decreased sperm counts and impaired sperm motility are present in a substantial proportion of men with varicocele. Elevations in the temperature of the affected testis, and increased spermatic vein estradiol (E2) concentrations have been found in some of these patients. To investigate the possibility that increases in temperature lead to a pattern of testicular steroidogenesis that results in increased E2 synthesis, we have examined the effects of temperature changes on the activities of four important testicular steroidogenic enzymes. 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), 17-hydroxylase (17-OH), 17,20-desmolase (17,20-D) and aromatase activities were measured in the microsomal fraction of rat, pig and horse testes. Incubations were performed at 34 degrees C, 36 degrees C, and 38 degrees C. The activities of all 4 enzymes increased with each 2 degrees C temperature elevation in roughly proportional amounts. We conclude that minor elevations in incubation temperature are associated with increases in the in vitro activity of four key testicular steroidogenic enzymes.
在患有精索静脉曲张的男性中，相当一部分存在精子数量减少和精子活力受损的情况。在其中一些患者中，已发现患侧睾丸温度升高以及精索静脉雌二醇（E2）浓度增加。为了研究温度升高是否会导致睾丸类固醇生成模式改变从而导致E2合成增加，我们研究了温度变化对四种重要睾丸类固醇生成酶活性的影响。在大鼠、猪和马睾丸的微粒体部分中测量了3β-羟基类固醇脱氢酶（3β-HSD）、17-羟化酶（17-OH）、17,20-裂解酶（17,20-D）和芳香化酶的活性。在34℃、36℃和38℃下进行孵育。随着温度每升高2℃，所有4种酶的活性大致成比例增加。我们得出结论，孵育温度的轻微升高与四种关键睾丸类固醇生成酶的体外活性增加有关。
