Kanter Mehmet, Aktas Cevat
Department of Histology and Embryology, Faculty of Medicine, Trakya University, 22030 Edirne, Turkey.
J Mol Histol. 2009 Apr;40(2):123-30. doi: 10.1007/s10735-009-9222-5. Epub 2009 May 31.
We have previously demonstrated that scrotal hyperthermia induce Leydig cell (LC) damage in short-term. The objectives of this pilot study were to investigate morphological changes and regulation of steroidogenesis on LC in long-term and the time of observation were extended to investigate whether the LC would eventually make a recovery after scrotal hyperthermia. The rats were randomly allotted into one of four groups: A (control), B (70 days after scrotal hyperthermia), C (105 days after scrotal hyperthermia), D (140 days after scrotal hyperthermia); each group contain seven animals. Scrotal hyperthermia was carried out in a thermostatically controlled water bath at 43 degrees C for 30 min once daily for six consecutive days. Control rats were treated in the same way, except the testes were immersed in a water bath maintained at 22 degrees C. Hyperthermia applied rats were sacrificed under 50 mg/kg ketamine anaesthesia after 70, 105 and 140 days, and biopsy materials of testes were obtained for light and electron microscopic examinations. Morphologically normal and the number of testosterone positive LC was significantly higher in 140 days after last heat than all other heat treatment groups. In heat treated groups, a dilated smooth endoplasmic reticulum, swollen mitochondria, and vanished mitochondrial cristae were observed. In the 140 days after scrotal hyperthermia, the severities of degenerative changes of LC were less than that observed in the other heat treated groups. We conclude that, scrotal hyperthermia cause morphological damaging and impaired steroidogenesis in LC and recovery of these findings were noted first time in 140 days after the last heat treatment.
我们之前已经证明，阴囊高温会在短期内诱导睾丸间质细胞（LC）损伤。本初步研究的目的是调查长期内LC的形态变化和类固醇生成调节情况，并延长观察时间以研究阴囊高温后LC是否最终会恢复。将大鼠随机分为四组之一：A组（对照组）、B组（阴囊高温后70天）、C组（阴囊高温后105天）、D组（阴囊高温后140天）；每组包含7只动物。在恒温43℃的水浴中进行阴囊高温处理，每天一次，持续30分钟，连续进行6天。对照组大鼠以相同方式处理，只是将睾丸浸入保持在22℃的水浴中。在70、105和140天后，对接受高温处理的大鼠在50mg/kg氯胺酮麻醉下进行处死，并获取睾丸活检材料进行光镜和电镜检查。末次高温处理后140天，形态正常且睾酮阳性的LC数量显著高于所有其他热处理组。在热处理组中，观察到滑面内质网扩张、线粒体肿胀和线粒体嵴消失。在阴囊高温后140天，LC的退行性变化严重程度低于其他热处理组。我们得出结论，阴囊高温会导致LC形态损伤和类固醇生成受损，并且在末次热处理后140天首次观察到这些结果的恢复。
