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腺病毒2型早期区域4信使核糖核酸中的剪接连接：多个剪接位点产生18至24种核糖核酸。

Splice junctions in adenovirus 2 early region 4 mRNAs: multiple splice sites produce 18 to 24 RNAs.

作者信息

Tigges M A, Raskas H J

出版信息

J Virol. 1984 Apr;50(1):106-17. doi: 10.1128/JVI.50.1.106-117.1984.

DOI:10.1128/JVI.50.1.106-117.1984
PMID:6336328
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC255589/
Abstract

We localized the splice junctions in adenovirus 2 early region 4 (E4) mRNAs. Processing of the E4 precursor RNA positioned the donor splice site of the 5' leader sequence adjacent to acceptor sites near the 5' ends of five of the six open reading regions in the E4 transcription unit. Of particular interest among the E4 mRNAs is an extensively spliced class which includes multiple species with sizes ranging from 1.1 to 0.75 kilobases (kb). Purified 1.1- to 0.75-kb mRNAs specified at least 10 polypeptides in vitro. We detected eight acceptor and two donor splice sites utilized in the deletion of the intron from the 3' portion of these mRNAs. E4 RNAs were isolated from the cytoplasm of infected cells at 5, 9, 12, and 18 h after infection. The E4 mRNAs were present throughout infection, but different members of the 1.1- to 0.7-kb class were predominant at each time assayed. Alternate splicing of the 3.0-kb E4 precursor RNA can generate as many as 25 mRNAs that encode at least 16 polypeptides.

摘要

我们对腺病毒2型早期区域4（E4）mRNA中的剪接位点进行了定位。E4前体RNA的加工过程使得5'前导序列的供体剪接位点与E4转录单元中六个开放阅读框中五个的5'端附近的受体位点相邻。在E4 mRNA中，特别引人关注的是一类经过广泛剪接的mRNA，其中包括多种大小在1.1至0.75千碱基（kb）之间的mRNA。纯化后的1.1至0.75 kb的mRNA在体外至少能编码10种多肽。我们检测到在这些mRNA的3'部分内含子缺失过程中使用了8个受体剪接位点和2个供体剪接位点。在感染后5、9、12和18小时从感染细胞的细胞质中分离出E4 RNA。在整个感染过程中都存在E4 mRNA，但在每次检测时，1.1至0.7 kb类别的不同成员占主导地位。3.0 kb E4前体RNA的可变剪接可产生多达25种mRNA，这些mRNA至少编码16种多肽。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8569/255589/17ae12345228/jvirol00133-0121-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8569/255589/e420f4e0001a/jvirol00133-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8569/255589/35ee1cb5ed0e/jvirol00133-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8569/255589/97c9e3c62575/jvirol00133-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8569/255589/d588052ed30f/jvirol00133-0120-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8569/255589/17ae12345228/jvirol00133-0121-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8569/255589/e420f4e0001a/jvirol00133-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8569/255589/35ee1cb5ed0e/jvirol00133-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8569/255589/97c9e3c62575/jvirol00133-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8569/255589/d588052ed30f/jvirol00133-0120-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8569/255589/17ae12345228/jvirol00133-0121-a.jpg

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本文引用的文献

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Cell. 1981 Feb;23(2):485-96. doi: 10.1016/0092-8674(81)90144-6.
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A 28K polypeptide is the translation product of 9 S RNA encoded by region 1A of adenovirus 2.
3型猪腺病毒早期区域4的特性分析
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Efficient dual transcomplementation of adenovirus E1 and E4 regions from a 293-derived cell line expressing a minimal E4 functional unit.来自表达最小E4功能单元的293衍生细胞系的腺病毒E1和E4区域的高效双转互补。
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Regulated splicing of adenovirus type 5 E4 transcripts and regulated cytoplasmic accumulation of E4 mRNA.5型腺病毒E4转录本的可变剪接及E4 mRNA在细胞质中的调控积累。
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Identification of adenovirus 2 early region 4 polypeptides by in vitro translation and tryptic peptide map analysis.通过体外翻译和胰蛋白酶肽图谱分析鉴定腺病毒2型早期区域4多肽。
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