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腺病毒2型E4区的组织与表达

Organization and expression of the E4 region of adenovirus 2.

作者信息

Rigolet M, Galibert F

出版信息

Nucleic Acids Res. 1984 Oct 25;12(20):7649-61. doi: 10.1093/nar/12.20.7649.

DOI:10.1093/nar/12.20.7649
PMID:6093046
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320191/
Abstract

The E4 region of Adenovirus 2 is a leftward transcribed part of the viral genome. Its nucleotide sequence has already been analysed. From this sequence several open reading frames were defined, which could be used in the coding of the E4 proteins. Using S1 digestion of mRNA-DNA hybrids a precise mapping of donor and acceptor sites was done. Their use in various combinations allows the synthesis of mRNAs, able to direct the synthesis of at least 7 polypeptides, ranging in size from 9K to 34K. Comparison of the sequences of the different acceptor sites indicates that they all conform to the consensus sequence. Analysis of the ATG surrounding sequence shows that initiator ATG may be positively selected according to Kozak's rule.

摘要

腺病毒2型的E4区域是病毒基因组向左转录的部分。其核苷酸序列已经过分析。从该序列中确定了几个开放阅读框，可用于E4蛋白的编码。通过对mRNA-DNA杂交体进行S1消化，对供体和受体位点进行了精确映射。它们以各种组合使用可合成能够指导至少7种多肽合成的mRNA，这些多肽大小从9K到34K不等。对不同受体位点序列的比较表明，它们均符合共有序列。对起始密码子周围序列的分析表明，起始ATG可能根据科扎克规则被正向选择。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c9b/320191/b0b9bf5f973e/nar00338-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c9b/320191/b0b9bf5f973e/nar00338-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c9b/320191/b0b9bf5f973e/nar00338-0046-a.jpg

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引用本文的文献

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本文引用的文献

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Control of adenovirus early gene expression: a class of immediate early products.腺病毒早期基因表达的调控：一类立即早期产物。
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Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.侧翼核苷酸在真核生物核糖体识别AUG起始密码子中的可能作用。
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Regulation of adenovirus-2 gene expression at the level of transcriptional termination and RNA processing.
腺病毒-2基因表达在转录终止和RNA加工水平上的调控。
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Expression of adenovirus-2 early region 4: assignment of the early region 4 polypeptides to their respective mRNAs, using in vitro translation.腺病毒2型早期区域4的表达：利用体外翻译将早期区域4多肽与其各自的信使核糖核酸进行对应
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Nucleotide sequence of adenovirus 2 DNA fragment encoding for the carboxylic region of the fiber protein and the entire E4 region.编码纤维蛋白羧基区域和整个E4区域的腺病毒2型DNA片段的核苷酸序列。
Nucleic Acids Res. 1981 Aug 25;9(16):4023-42. doi: 10.1093/nar/9.16.4023.
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J Virol. 1983 Feb;45(2):514-23. doi: 10.1128/JVI.45.2.514-523.1983.
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