Suppr超能文献

[拉普拉塔河用于休闲娱乐水域中沙门氏菌的分离与定量分析]

[Isolation and quantification of Salmonella in the waters of the Río de la Plata destined for recreation].

作者信息

Monticelli L S, Lasta J A, Gariboglio M A

机构信息

Instituto de Limnología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, CONICET, Argentina.

出版信息

Rev Argent Microbiol. 1984;16(1):1-10.

PMID:6336358
Abstract

Thirty three samples from recreational waters from different areas of Río de la Plata were tested for Salmonella (Fig. 1). A 200 ml sample filtered through a diatom bed as well as 2 and 20 ml samples without filtering were studied using a simple and economical technique. The isolation probability was directly dependent on the water volume analyzed. Best results were obtained when tetrathionate broth with novobiocin and Salmonella shigella agar were used (Table 3). Salmonella was isolated from 36% of the samples studied and 12 serotypes were identified (Table 5). The relation between the NPM of each bacteriological indicator used and the NPM of Salmonella are shown in Table 4. Correlation values were calculated by relating mean point logarithms of the classes in Table 4 with the isolation percentages of Salmonella for each class. These values were low, except for fecal Streptococcus (R2 = 0.94). The high density of fecal coliforms found in Bagliardi and Pescadores Beaches (rarely less than 10.000 bacteria/100 ml) and the incidence of Salmonella, lead us to conclude that these waters are unsafe for bathing and swimming, specially for children, according with the international recommendation on the quality of recreational waters. Waters with the characteristics described here should be periodically analyzed for Salmonella concentration. The lack of correlation values between the MPN of total and fecal coliforms and the MPN of Salmonella, also supports this advise.

摘要

对来自拉普拉塔河不同区域的33份娱乐用水样本进行了沙门氏菌检测(图1)。使用一种简单且经济的技术对通过硅藻床过滤的200毫升样本以及2毫升和20毫升未过滤样本进行了研究。分离概率直接取决于分析的水量。当使用含新生霉素的四硫磺酸盐肉汤和沙门氏菌志贺氏菌琼脂时,获得了最佳结果(表3)。在所研究的样本中,36%分离出了沙门氏菌,并鉴定出12种血清型(表5)。表4显示了所使用的每种细菌学指标的最可能数(MPN)与沙门氏菌的MPN之间的关系。通过将表4中各等级的平均点对数与每个等级沙门氏菌的分离百分比相关联来计算相关值。除粪链球菌外(R2 = 0.94),这些值都很低。在Bagliardi海滩和Pescadores海滩发现的高密度粪大肠菌群(很少低于10000个细菌/100毫升)以及沙门氏菌的发生率,使我们得出结论,根据关于娱乐用水质量的国际建议,这些水域对于游泳和沐浴是不安全的,特别是对儿童而言。具有此处所述特征的水域应定期分析沙门氏菌浓度。总大肠菌群和粪大肠菌群的MPN与沙门氏菌的MPN之间缺乏相关值,这也支持了这一建议。

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验