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[皮苏纳病毒在大鼠胚胎培养物中的增殖]

[Propagation of Pixuna virus in rat embryo cultures].

作者信息

Sampieri L, Sartori T, Paglini S

机构信息

Instituto de Virología Dr. José M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.

出版信息

Rev Argent Microbiol. 1984;16(1):45-8.

PMID:6336360
Abstract

The behaviour of cell cultures from 11 to 18 days-old Swiss albino mouse embryo infected with Pixuna virus was studied. Eleven day-old embryo cultures showed to be a permissive system supporting a productive and cytocidal infection. In contrast, the 13 to 18 day-old embryo cultures were also permissive and productive but no evidence of cytopathic effect was observed. D-glucosamine did not affect the infectivity titer of the Pixuna virus when 11 day-old embryo cultures were used. In these cultures the eclipse phase was observed between 15 and 90 min post-absorption (Figure 1).

摘要

研究了感染皮克纳病毒的11至18日龄瑞士白化小鼠胚胎的细胞培养行为。11日龄胚胎培养物显示为支持生产性和细胞杀伤性感染的允许系统。相比之下,13至18日龄胚胎培养物也是允许的且具有生产性,但未观察到细胞病变效应的证据。当使用11日龄胚胎培养物时,D-葡萄糖胺不影响皮克纳病毒的感染滴度。在这些培养物中,吸附后15至90分钟观察到隐蔽期(图1)。

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