Muller H K, Pye D W, Martin C L, Kimpton W G
Int Arch Allergy Appl Immunol. 1983;70(1):65-70. doi: 10.1159/000233275.
Tuberculin anergy was demonstrated in a number of clinically normal individuals who, on being immunized with Bacillus Calmette-Guérin (BCG), failed to demonstrate a positive Mantoux skin test. Lymphoid cells from these anergic subjects when stimulated with purified protein derivative (PPD) in vitro did not produce macrophage migration inhibition factor (MIF). Lymphocyte transformation studies demonstrated that while Mantoux-positive, non-BCG immunized Mantoux-negative and anergic individuals could all undergo blast cell transformation in the presence of PHA, only lymphoid cells from Mantoux-positive and a proportion of anergic people were capable of blast cell transformation when cultured with PPD. These results suggest that more than one mechanism may account for tuberculin anergy in clinically normal people operating at the level of lymphokine production and in some cases lymphocyte transformation.
在一些临床正常个体中证实存在结核菌素无反应性，这些个体在用卡介苗（BCG）免疫后，未能显示出阳性的结核菌素皮肤试验结果。这些无反应性受试者的淋巴细胞在体外受到纯化蛋白衍生物（PPD）刺激时，不会产生巨噬细胞移动抑制因子（MIF）。淋巴细胞转化研究表明，虽然结核菌素试验阳性、未接种卡介苗且结核菌素试验阴性以及无反应性的个体在有植物血凝素（PHA）存在的情况下均可发生母细胞转化，但只有来自结核菌素试验阳性个体以及一部分无反应性个体的淋巴细胞在用PPD培养时能够发生母细胞转化。这些结果表明，在临床正常人群中，结核菌素无反应性可能由多种机制导致，这些机制作用于淋巴因子产生水平，在某些情况下也作用于淋巴细胞转化。
