rfaD基因编码ADP-L-甘油-D-甘露庚糖-6-表异构酶。这是一种脂多糖核心生物合成所需的酶。
The rfaD gene codes for ADP-L-glycero-D-mannoheptose-6-epimerase. An enzyme required for lipopolysaccharide core biosynthesis.
作者信息
Coleman W G
出版信息
J Biol Chem. 1983 Feb 10;258(3):1985-90.
PMID:6337148
Abstract
The rfaD gene product, ADP-L-glycero-D-mannoheptose-6-epimerase, is necessary for the conversion of ADP-D-glycero-D-mannoheptose to ADP-L-glycero-D-mannoheptose. The nucleotide ADP-D-glycero-D-mannoheptose accumulates in rfaD mutant strains. Two chimeric colicin E1 plasmids carrying the coding sequence of the rfaD+ locus have been selected and shown to complement the rfaD phenotype. These clones also result in an amplification of ADP-L-glycero-D-mannoheptose-6-epimerase activity.
摘要
rfaD基因产物，ADP-L-甘油-D-甘露庚糖-6-表异构酶，对于将ADP-D-甘油-D-甘露庚糖转化为ADP-L-甘油-D-甘露庚糖是必需的。核苷酸ADP-D-甘油-D-甘露庚糖在rfaD突变菌株中积累。已筛选出两个携带rfaD+基因座编码序列的嵌合大肠杆菌素E1质粒，并证明它们可互补rfaD表型。这些克隆还导致ADP-L-甘油-D-甘露庚糖-6-表异构酶活性的增加。
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