Kutner S, Ginsburg H, Cabantchik Z I
J Cell Physiol. 1983 Feb;114(2):245-51. doi: 10.1002/jcp.1041140215.
The development of the malaria parasite Plasmodium falciparum in human red blood cells induces parasite-dependent perturbations in the permselectivity properties of the host cell membrane. The changes appear as parasites develop from ring to the trophozoite stage and persist during schizogony. In the present work we assessed the permeability changes of the infected cells to anionic substances by the use of radioactive and fluorescent probes. Our data show that i) covalent binding probes, such as diisothiocyano ditritiostilbene disulfonic acid [3H]H2DIDS, which are virtually impermeant to normal red blood cells, became markedly permeant to trophozoites and schizonts, as evidenced by high labeling of intracellular hemoglobin; ii) permeation of the fluorescent anion transport substrate NBD-taurine, measured in the efflux mode, was very rapid and substantially enhanced in parasitized erythrocytes, as compared with noninfected cells; iii) this efflux could not be blocked by H2DIDS, which is a specific inhibitor of anion transport in normal red blood cells; iv) permeation of anionic probes was temperature dependent (Ea:11 +/- 1 kcal/mole); and v) could be blocked by nonspecific transport inhibitors that are known to interact with membrane lipids. The appearance of a new permeation pathway in the host cell membrane of trophozoites is associated with structural modification of the host cell membrane matrix.
恶性疟原虫在人类红细胞中的发育会引发宿主细胞膜通透选择性特性的寄生虫依赖性扰动。这些变化在寄生虫从环状体发育到滋养体阶段时出现，并在裂殖生殖过程中持续存在。在本研究中，我们通过使用放射性和荧光探针评估了受感染细胞对阴离子物质的通透性变化。我们的数据表明：i）共价结合探针，如二异硫氰基二氚代芪二磺酸[³H]H₂DIDS，对正常红细胞几乎不通透，但对滋养体和裂殖体却具有明显的通透性，细胞内血红蛋白的高标记证明了这一点；ii）以流出模式测量的荧光阴离子转运底物NBD - 牛磺酸的渗透非常迅速，与未感染细胞相比，在寄生红细胞中显著增强；iii）这种流出不能被H₂DIDS阻断，H₂DIDS是正常红细胞中阴离子转运的特异性抑制剂；iv）阴离子探针的渗透与温度有关（活化能：11±1千卡/摩尔）；v）可以被已知与膜脂质相互作用的非特异性转运抑制剂阻断。滋养体宿主细胞膜中出现新的渗透途径与宿主细胞膜基质的结构改变有关。
