福氏志贺氏菌热不稳定抗原在HeLa细胞侵袭中的作用。

Role of heat labile antigens of Shigella flexneri in HeLa cell invasion.

作者信息

Bhogale S R, Sharma K D, Kamat R S

出版信息

J Med Microbiol. 1983 Feb;16(1):37-43. doi: 10.1099/00222615-16-1-37.

Abstract

In studies of the role of surface antigens of Shigella flexneri in HeLa cell invasion, three antisera were employed to block the invasion. Antisera against live (ALS) and boiled (ABS) S. flexneri blocked invasion very effectively. Reduction in the numbers of intracellular shigellae was always accompanied by reduction in the number adherent to the cells, indicating the importance of adhesion in the invasive process. Anti-live absorbed antiserum (ALAS) prepared by exhaustive absorption of ALS with boiled S. flexneri blocked adhesion and invasion at dilutions of 20 or 50; the efficiency of the absorption was indicated by absence of agglutinating and anti-lipopolysaccharide (LPS) antibodies. S. flexneri LPS did not block adhesion and invasion even at a concentration of 1.0 mg/ml. Hence it was concluded that heat-labile surface antigens are important in adhesion and invasion of HeLa cells by S. flexneri. Antiserum against heat stable antigen (ABS) probably blocks adhesion by steric hindrance.

摘要

在关于福氏志贺菌表面抗原在HeLa细胞侵袭中作用的研究中，使用了三种抗血清来阻断侵袭。抗活福氏志贺菌（ALS）和抗煮沸福氏志贺菌（ABS）的抗血清能非常有效地阻断侵袭。细胞内志贺菌数量的减少总是伴随着黏附于细胞的志贺菌数量的减少，这表明黏附在侵袭过程中很重要。用煮沸的福氏志贺菌对ALS进行彻底吸收制备的抗活吸收抗血清（ALAS）在20倍或50倍稀释时能阻断黏附和侵袭；凝集抗体和抗脂多糖（LPS）抗体的缺失表明了吸收的效率。即使在浓度为1.0mg/ml时，福氏志贺菌LPS也不能阻断黏附和侵袭。因此得出结论，热不稳定表面抗原在福氏志贺菌对HeLa细胞的黏附和侵袭中很重要。抗热稳定抗原的抗血清（ABS）可能通过空间位阻来阻断黏附。