Hubbard J W, Cox R H, Lawler J E, Blank M L, Snyder F
Life Sci. 1983 Jan 17;32(3):221-32. doi: 10.1016/0024-3205(83)90034-6.
Fourteen 23 week old male spontaneously hypertensive rats (SHR) were randomly divided into saline control or phospholipid (I-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine) treatment groups. Four weeks of baseline systolic blood pressure (SBP) and heart rate (HR) measurements were determined via tail plethysmography. On week 25 of the baseline period a 1.5 ml blood sample was taken by tail clip for analysis of norepinephrine (NE), epinephrine (E), and plasma renin activity (PRA). On the following week, a single injection of phospholipid (11 ug/kg, s.c.) was given to the experimental animals following baseline SBP and HR determinations. A similar procedure was employed for control subjects, except they received an injection of normal saline (0.5 ml, s.c.). Systolic BP and HR responses were monitored for 24 minutes following the injection. A 1.5 ml blood sample was taken at the end of the 4th minute for NE, E, and PRA assays. A significant drop in SBP (202 +/- 5 mmHg to 124 +/- 6 mmHg) and an increase in HR (431 +/- 17 bpm to 519 +/- 21 bpm) were observed for experimental animals, but not for control subjects. Plasma NE increased significantly (446 +/- 42 pg/ml to 1099 +/- 77 pg/ml), but E remained unchanged following treatment with the phospholipid. Plasma renin activity increased for both groups, but this change was only significant for the experimental group (18.1 +/- 5.7 ng Al/ml/hr to 34.3 +/- 3.6 ng Al/ml/hr). Thus, it appears that I-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine is a potent antihypertensive vasodilating agent which stimulates baroreceptor mediated sympathetic discharge to the heart and kidneys of the SHR.
将14只23周龄的雄性自发性高血压大鼠（SHR）随机分为生理盐水对照组或磷脂（1-十六烷基-2-乙酰基-sn-甘油-3-磷酸胆碱）治疗组。通过尾容积描记法测定四周的基线收缩压（SBP）和心率（HR）。在基线期的第25周，通过尾夹采集1.5 ml血样，用于分析去甲肾上腺素（NE）、肾上腺素（E）和血浆肾素活性（PRA）。在接下来的一周，在测定基线SBP和HR后，给实验动物单次注射磷脂（11 μg/kg，皮下注射）。对对照组采用类似的程序，不同的是他们接受了生理盐水注射（0.5 ml，皮下注射）。注射后监测收缩压和心率反应24分钟。在第4分钟末采集1.5 ml血样用于NE、E和PRA检测。实验动物的SBP显著下降（从202±5 mmHg降至124±6 mmHg），HR增加（从431±17次/分钟增至519±21次/分钟），而对照组未出现这种情况。血浆NE显著增加（从446±42 pg/ml增至1099±77 pg/ml），但用磷脂治疗后E保持不变。两组的血浆肾素活性均增加，但这种变化仅在实验组有统计学意义（从18.1±5.7 ng Al/ml/hr增至34.3±3.6 ng Al/ml/hr）。因此，1-十六烷基-2-乙酰基-sn-甘油-3-磷酸胆碱似乎是一种有效的降压血管扩张剂，它刺激压力感受器介导的对SHR心脏和肾脏的交感神经放电。
