Kitamura Y, Yokoyama M, Sonoda T, Mori K J
Radiat Res. 1983 Jan;93(1):147-56.
Although tissue mast cells are derived from the bone marrow, some descendants of bone marrow-derived precursors retain the ability to proliferate and differentiate into mast cells even after localization in the skin. The purpose of the present study was to determine the D0 values for mast-cell precursors in the bone marrow and those localized in the skin. Bone marrow cells were removed from (WB X C57BL/6)F1-+/+ mice after various doses of irradiation and injected into the skin of the congenic W/Wv mice which were genetically without mast cells. Radiosensitivity of mast-cell precursors in the bone marrow was evaluated by determining the proportion of the injection sites at which mast cells did not appear. For the assay of the radiosensitivity of mast-cell precursors localized in the skin, pieces of skin were removed from beige C57BL/6 (bgJ/bgJ. Chediak-Higashi syndrome) mice after various doses of irradiation and grafted onto the back of the normal C57BL/6 mice. Radiosensitivity of mast-cell precursors in the skin was evaluated by determining the decrease of beige-type mast cells which possessed giant granules. Mast-cell precursors in the bone marrow were much more radiosensitive than those localized in the skin. D0 value was about 100 rad for the former and about 800 rad for the latter.
尽管组织肥大细胞起源于骨髓，但骨髓来源的前体细胞的一些后代即使在定位于皮肤后仍保留增殖并分化为肥大细胞的能力。本研究的目的是确定骨髓中肥大细胞前体细胞以及定位于皮肤中的肥大细胞前体细胞的D0值。在给予不同剂量的辐射后，从（WB×C57BL/6）F1-+/+小鼠中取出骨髓细胞，并将其注射到基因上无肥大细胞的同基因W/Wv小鼠的皮肤中。通过确定未出现肥大细胞的注射部位的比例来评估骨髓中肥大细胞前体细胞的放射敏感性。为了测定定位于皮肤中的肥大细胞前体细胞的放射敏感性，在给予不同剂量的辐射后，从米色C57BL/6（bgJ/bgJ，切迪阿克-东综合征）小鼠身上取下皮肤碎片，并移植到正常C57BL/6小鼠的背部。通过确定具有巨大颗粒的米色型肥大细胞的减少来评估皮肤中肥大细胞前体细胞的放射敏感性。骨髓中的肥大细胞前体细胞比定位于皮肤中的肥大细胞前体细胞对辐射更敏感。前者的D0值约为100拉德，后者约为800拉德。
