Sonoda T, Ohno T, Kitamura Y
J Cell Physiol. 1982 Jul;112(1):136-40. doi: 10.1002/jcp.1041120120.
When hematopoietic cells of congenic +/+ mice were injected into the skin of genetically mast-cell-depleted (WB X C57BL/6)F1-W/Wv mice, mast cells appeared at the injection site. The donor origin of developing mast cells was confirmed by using giant granules of C57BL/6-bgl/bgl mice as a marker. When the number of injected cells was decreased, the proportion of injection sites at which mast cells did not appear increased according to the expected frequency of null response in a Poisson distribution. Therefore, such proportions were used to calculate the concentration of mast-cell precursors in the bone marrow, spleen, and peripheral blood. The relative concentration of mast-cell precursors in these tissues was similar to that of spleen-colony-forming cells. The present method seems useful as a semiquantitative in vivo assay for a population of progenitor cells which are committed to differentiate into mast cells.
将同基因+/+小鼠的造血细胞注射到基因性肥大细胞缺陷的（WB×C57BL/6）F1-W/Wv小鼠的皮肤中时，肥大细胞出现在注射部位。通过使用C57BL/6-bgl/bgl小鼠的巨大颗粒作为标志物，证实了发育中的肥大细胞的供体来源。当注射的细胞数量减少时，根据泊松分布中无效反应的预期频率，肥大细胞未出现的注射部位比例增加。因此，利用这些比例来计算骨髓、脾脏和外周血中肥大细胞前体细胞的浓度。这些组织中肥大细胞前体细胞的相对浓度与脾集落形成细胞的相似。本方法似乎可作为一种半定量体内试验，用于检测致力于分化为肥大细胞的祖细胞群体。
