Imamura M, Miyazaki T, Okabe M, Sakurada K, Musashi M, Kawamura K, Hatakeyama M
Transplantation. 1983 Jan;35(1):19-24. doi: 10.1097/00007890-198301000-00005.
Radiation chimeras in mice were induced by intrasplenic injection of allogeneic bone marrow cells instead of intravenous injection. Interestingly, the survival time in X-irradiated BALB/c mice inoculated intrasplenically (i.s.) with bone marrow cells from C3H/He mice was markedly prolonged as compared with that in X-irradiated BALB/c mice inoculated i.v. with bone marrow cells from C3H/He mice. However, when C57BL/6 mice were used as donors, a significant difference between i.s. injection and i.v. injection has not been found in survival time at 60 days after X irradiation. On the contrary, when bone marrow cells from BALB/c or C57BL/6 mice were injected into X-irradiated C3H/He mice, i.s. injection gave longer survival days to recipients than did i.v. injection. Based on testing their chimerism, it was suggested that lymphoid cells of donor origin were predominantly identified in almost all BALB/c or C3H/He recipients which were inoculated i.s. with bone marrow cells from C57BL/6 mice. However, somewhat incomplete chimerism was observed when the C3H/He to BALB/c donor-recipient combination was used and vice versa.
通过脾内注射同种异体骨髓细胞而非静脉注射来诱导小鼠的辐射嵌合体。有趣的是，与经X射线照射后静脉注射C3H/He小鼠骨髓细胞的BALB/c小鼠相比，经X射线照射后脾内接种C3H/He小鼠骨髓细胞的BALB/c小鼠的存活时间显著延长。然而，当使用C57BL/6小鼠作为供体时，在X射线照射后60天的存活时间方面，脾内注射和静脉注射之间未发现显著差异。相反，当将BALB/c或C57BL/6小鼠的骨髓细胞注射到经X射线照射的C3H/He小鼠中时，脾内注射给予受体的存活天数比静脉注射更长。基于对它们嵌合状态的检测，表明在几乎所有经脾内接种C57BL/6小鼠骨髓细胞的BALB/c或C3H/He受体中，主要鉴定出了供体来源的淋巴细胞。然而，当使用C3H/He到BALB/c的供体-受体组合时，观察到嵌合状态有些不完全，反之亦然。
