Leech S H, Mitchison N A
Eur J Immunol. 1976 Nov;6(11):810-6. doi: 10.1002/eji.1830061111.
Mice tolerized by low doses of bovine IgG (BGG), immunized with arsanilated (ARS) conjugates and challenged with ARS-BGG show an augmented anti-BGG antibody response compared with controls immunized with nonconjugated carrier. Conditions optimal for generating hapten help are explored and suggest that the phenomenon is mediated by hapten-specific helper T cells. During exploration of these conditions it became apparent that the response was suppression rather than augmentation with ARS, under certain circumstances, and with 2,4-dinitrophenyl, 4-hydroxy-5-iodo-3-nitro-phenylacetyl (NIP) and sulfanilic acid, under all conditions tested. Especially important in determining the outcome of the interaction was the type of adjuvant used for hapten priming. The significance of these observations is discussed in relationship to the balance between negative and positive cooperation.
用低剂量牛IgG（BGG）致敏的小鼠，用偶氮砷酸（ARS）偶联物免疫并接受ARS - BGG攻击，与用未偶联载体免疫的对照相比，其抗BGG抗体反应增强。研究了产生半抗原辅助的最佳条件，并表明该现象由半抗原特异性辅助性T细胞介导。在探索这些条件的过程中，很明显在某些情况下，ARS以及在所有测试条件下的2,4 - 二硝基苯基、4 - 羟基 - 5 - 碘 - 3 - 硝基 - 苯基乙酰基（NIP）和对氨基苯磺酸，反应是抑制而非增强。在确定相互作用结果时特别重要的是用于半抗原致敏的佐剂类型。结合阴性和阳性协同作用之间的平衡讨论了这些观察结果的意义。
