蛋白酶的双底物平板扩散测定法。
Dual-substrate plate diffusion assay for proteases.
作者信息
Montville T J
出版信息
Appl Environ Microbiol. 1983 Jan;45(1):200-4. doi: 10.1128/aem.45.1.200-204.1983.
Abstract
A plate diffusion assay for endopeptidases was developed. Proteases applied to plates containing 1% casein, 1% gelatin, and 1.5% agar caused distinct zones reminiscent of immunoprecipitation bands. The diameter of the zones was linearly proportional to the log of the enzyme activity applied over a range from 0.01 to greater than 100 IU/ml.
摘要
开发了一种用于内肽酶的平板扩散测定法。将蛋白酶应用于含有1%酪蛋白、1%明胶和1.5%琼脂的平板上，会产生类似于免疫沉淀带的明显区域。在0.01至大于100 IU/ml的范围内，这些区域的直径与所施加酶活性的对数呈线性比例关系。
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