酵母苯丙氨酸转移核糖核酸中锌（II）结合位点的X射线衍射研究。锌在嘌呤-嘌呤序列中与鸟嘌呤的优先结合。
X-ray diffraction study of the zinc(II) binding sites in yeast phenylalanine transfer RNA. Preferential binding of zinc to guanines in purine-purine sequences.
作者信息
Rubin J R, Wang J, Sundaralingam M
出版信息
Biochim Biophys Acta. 1983 Mar 15;756(1):111-8. doi: 10.1016/0304-4165(83)90030-2.
PMID:6337651
Abstract
An X-ray diffraction study of a zinc(II) complex of tRNAPhe from yeast reveals the present of five zinc-binding sites on the tRNA molecule. Two of the cooperatively bound Mg2+ in the native tRNA structure are replaced by Zn2+. The remaining sites involve direct coordination of zinc to the N7 position of tRNA guanine bases, G15, G43 and HG45. Thus, zinc displays a high specificity for binding to guanine bases in purine-purine sequences.
摘要
对来自酵母的苯丙氨酸转运核糖核酸（tRNAPhe）的锌（II）配合物进行的X射线衍射研究揭示了tRNA分子上存在五个锌结合位点。天然tRNA结构中两个协同结合的Mg2+被Zn2+取代。其余位点涉及锌与tRNA鸟嘌呤碱基G15、G43和HG45的N7位置直接配位。因此，锌对嘌呤-嘌呤序列中的鸟嘌呤碱基具有高度的结合特异性。
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