Moore J V, Maunda K K
Br J Radiol. 1983 Mar;56(663):193-9. doi: 10.1259/0007-1285-56-663-193.
In the intestinal crypt microcolony assay, a "surviving fraction" of whole crypts is calculated conventionally by dividing the number of regenerating crypts three to four days after treatment by the number of crypts in untreated animals, both measured around a complete intestinal circumference in transverse sections of gut. We show that in relation to the mesenteric attachment of the gut, crypts in different regions of this circumference differ in their survival characteristics after radiation or mechlorethamine hydrochloride ("HN2"). Crypts associated with Peyer's patches are resistant to both agents (large initial shoulder, shallow slope). The mode of administration of HN2 (IP vs. IV) influences the shape of the crypt survival curve. Differences in size of shoulder and slope affect the estimate of numbers of microcolony-forming cells per crypt (A). For radiation, this number can vary sevenfold, depending on the region of circumference chosen for analysis. Different agents of assay result in radically different values for A.
在肠道隐窝微集落试验中，整个隐窝的“存活分数”通常通过将处理后三到四天再生隐窝的数量除以未处理动物隐窝的数量来计算，两者均在肠道横切面上围绕完整的肠周长进行测量。我们发现，相对于肠道的肠系膜附着部位，该周长不同区域的隐窝在接受辐射或盐酸氮芥（“HN2”）后的存活特性存在差异。与派尔集合淋巴结相关的隐窝对这两种试剂均具有抗性（初始肩部大，斜率浅）。HN2的给药方式（腹腔注射与静脉注射）会影响隐窝存活曲线的形状。肩部和斜率大小的差异会影响每个隐窝中微集落形成细胞数量的估计值（A）。对于辐射，该数值可能会相差七倍，具体取决于选择用于分析的周长区域。不同的试验试剂会导致A值截然不同。
