Rubinstein R, Polson A
Intervirology. 1983;19(1):16-25. doi: 10.1159/000149332.
The role played by the gut juice of insects in the infective process of insect viruses was examined. Analysis of larval gut extract of Heliothis armigera by SDS-PAGE revealed protease activity associated with components of molecular weights 48,000 and 94,000. Proteases were found to be associated with occlusion bodies and virions of both nuclear polyhedrosis virus (NPV) and cytoplasmic polyhedrosis virus (CPV) infecting H. armigera. CPV occlusion bodies were dissolved by gut juice extract at pH 8.0, trypsin and chymotrypsin at pH 8.0, and carbonate-chloride solution at pH 10.5. Trypsin treatment was selective for occlusion bodies of CPV at pH 8.0, whereas solutions more alkaline than pH 10.0 without added enzymes were adequate to digest NPV occlusion bodies. This property was used to identify and separate the two types of viruses from a mixed infection. Gut extract proteases have characteristics similar to those of trypsin.
研究了昆虫肠液在昆虫病毒感染过程中所起的作用。通过SDS-PAGE对棉铃虫幼虫肠提取物进行分析，发现蛋白酶活性与分子量为48,000和94,000的组分相关。已发现蛋白酶与感染棉铃虫的核型多角体病毒（NPV）和质型多角体病毒（CPV）的包涵体及病毒粒子相关。CPV包涵体在pH 8.0的肠液提取物、pH 8.0的胰蛋白酶和胰凝乳蛋白酶以及pH 10.5的碳酸盐 - 氯化物溶液作用下会溶解。在pH 8.0时，胰蛋白酶处理对CPV包涵体具有选择性，而pH高于10.0且未添加酶的溶液足以消化NPV包涵体。利用这一特性从混合感染中鉴定和分离这两种病毒。肠提取物蛋白酶具有与胰蛋白酶相似的特性。
