通过局部诱变分离大肠杆菌的条件致死突变体突变株。
Isolation of conditional lethal mutator mutants of Escherichia coli by localized mutagenesis.
作者信息
Maki H, Horiuchi T, Sekiguchi M
出版信息
J Bacteriol. 1983 Mar;153(3):1361-7. doi: 10.1128/jb.153.3.1361-1367.1983.
Abstract
By using localized mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, we isolated 39 temperature-sensitive growth mutants that exhibited high mutability when the bacteria were grown at the permissive temperature. Two of the mutations, dnaQ186 and dnaQ231, were shown to be new alleles of the dnaQ gene by genetic mapping and complementation tests with the dnaQ49 mutation previously isolated. They shared common properties with the dnaQ49 strain, but their mutator activity was not temperature dependent. The dnaQ mutants exhibited increased sensitivity to inhibitors of DNA gyrase and to DNA intercalating and alkylating agents.
摘要
通过使用N-甲基-N'-硝基-N-亚硝基胍进行定位诱变，我们分离出了39个温度敏感型生长突变体，当细菌在允许温度下生长时，这些突变体表现出高突变率。通过遗传定位和与先前分离的dnaQ49突变进行互补测试，发现其中两个突变dnaQ186和dnaQ231是dnaQ基因的新等位基因。它们与dnaQ49菌株具有共同特性，但它们的诱变活性不依赖于温度。dnaQ突变体对DNA回旋酶抑制剂以及DNA嵌入剂和烷化剂表现出更高的敏感性。
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