细菌中的非染色体抗生素抗性：R 因子 DNA 对大肠杆菌的遗传转化
Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
作者信息
Cohen S N, Chang A C, Hsu L
出版信息
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4. doi: 10.1073/pnas.69.8.2110.
Abstract
Transformation of E. coli cells treated with CaCl(2) to multiple antibiotic resistance by purified R-factor DNA is reported. Drug resistance is expressed in a small fraction of the recipient bacterial population almost immediately after uptake of DNA, but full genetic expression of resistance requires subsequent incubation in drugfree medium before antibiotic challenge. Transformed bacteria acquire a closed circular, transferable DNA species having the resistance, fertility, and sedimentation characteristics of the parent R factor. Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R-factor DNA in this system.
摘要
据报道，用纯化的R因子DNA可使经氯化钙处理的大肠杆菌细胞转变为多重抗生素抗性。几乎在摄取DNA后，一小部分受体细菌群体中就立即表现出耐药性，但在抗生素攻击之前，耐药性的完全基因表达需要随后在无药物培养基中培养。转化后的细菌获得一种闭合环状、可转移的DNA分子，其具有亲本R因子的抗性、育性和沉降特性。R因子DNA的共价闭合、连环和开放（带切口）环状形式在转化中均有效，但变性和超声处理会消除该系统中R因子DNA的转化能力。
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