Van Oudenaren A, Haaijman J J, Benner R
J Immunol Methods. 1983;56(1):117-24. doi: 10.1016/0022-1759(83)90054-6.
This paper reports a comparative study on the sensitivity of the protein A plaque assay and the cytoplasmic immunofluorescence assay for detection of immunoglobulin (Ig) producing cells in cell suspensions of murine lymphoid organs. The protein A plaque assay was found to detect as many or several times more Ig producing cells than the immunofluorescence assay, depending on the age and antigenic load of the mice, and upon the Ig class and organ studied.
本文报道了一项关于蛋白A空斑试验和细胞质免疫荧光试验检测小鼠淋巴器官细胞悬液中免疫球蛋白（Ig）产生细胞敏感性的比较研究。结果发现，根据小鼠的年龄、抗原负荷以及所研究的Ig类别和器官不同，蛋白A空斑试验检测到的Ig产生细胞数量与免疫荧光试验相当，甚至比后者多几倍。
