Makonkawkeyoon S, Vithayasai V
J Immunol Methods. 1983 Sep 16;62(3):365-71. doi: 10.1016/0022-1759(83)90181-3.
A pre-amplified reverse hemolytic plaque assay has been developed. Sheep red blood cells (SRBC) were coated with staphylococcal protein A (SPA) by the chromic chloride method. The protein A-coated SRBC (SPA-SRBC) was then pre-amplified with an appropriate amount of human class-specific Ig. The pre-amplified Ig-SPA-SRBC was used to detect class-specific Ig-producing cells. It was found that this pre-amplified reverse hemolytic plaque assay gave clearer, larger and more numerous hemolytic plaques which were easy to count and thus gave more accurate results.
已开发出一种预扩增反向溶血空斑试验。采用氯化铬法将羊红细胞（SRBC）用葡萄球菌蛋白A（SPA）包被。然后用适量的人类类别特异性免疫球蛋白对包被蛋白A的SRBC（SPA - SRBC）进行预扩增。预扩增的免疫球蛋白 - SPA - SRBC用于检测产生类别特异性免疫球蛋白的细胞。结果发现，这种预扩增反向溶血空斑试验产生的溶血空斑更清晰、更大且数量更多，易于计数，因此结果更准确。
