López A F, Ribeiro dos Santos R, Sanderson C J
Parasite Immunol. 1983 Jan;5(1):77-84. doi: 10.1111/j.1365-3024.1983.tb00725.x.
Eosinophils and neutrophils are shown to be cytotoxic against two syngeneic mouse cell lines cells when these are coated with T. cruzi antigen and anti-T. cruzi antibody. Activity is detected within 5 h of incubation. Highest levels of cytotoxicity are obtained at antibody dilutions of 1:100 and 1:1000, while antiserum at 1:10 is shown to be inhibitory. Eosinophils show significant activity at an effector to target ratio of 5:1. No cytotoxicity occurs in the absence of either antigen, antibody or effector cells. This phenomenon may be a model for the tissue destruction in acute T. cruzi infection, where the lysis of trypanosomes may lead to antigen coating of host cells, followed by antibody-dependent granulocyte-mediated cytotoxicity of the host cells.
当两种同基因小鼠细胞系细胞被克氏锥虫抗原和抗克氏锥虫抗体包被时，嗜酸性粒细胞和中性粒细胞对它们具有细胞毒性。在孵育5小时内可检测到活性。在抗体稀释度为1:100和1:1000时可获得最高水平的细胞毒性，而1:10的抗血清则表现出抑制作用。嗜酸性粒细胞在效应细胞与靶细胞比例为5:1时表现出显著活性。在没有抗原、抗体或效应细胞的情况下不会发生细胞毒性。这种现象可能是急性克氏锥虫感染中组织破坏的一个模型，其中锥虫的裂解可能导致宿主细胞的抗原包被，随后是抗体依赖性粒细胞介导的宿主细胞细胞毒性。
