Sanderson C J, de Souza W
J Cell Sci. 1979 Jun;37:275-86. doi: 10.1242/jcs.37.1.275.
The interaction between trypomastigotes of T. cruzi and purified rat eosinophils, neutrophils and macrophages has been studied. Macrophages induced by dextran, became infected and the parasites multiplied as amastigotes, eventually being released as trypomastigotes into the medium. Eosinophils show an antibody-dependent phagocytosis, while neutrophil phagocytosis is enhanced by antibody. Electron microscopy showed that once inside the granulocyte phagosome, the trypomastigotes transform to amastigotes as a prelude to degeneration. This occurs as material apparently of granule origin accumulates in the phagosome. Eosinophils have the capacity to eject incompletely degenerated amastigotes into the medium. This phenomenon is discussed in relation to previous time-lapse cinematography studies of the lysis of chick erythrocytes by eosinophils, and reports that eosinophils release granule contents by means of a secretory vesicle.
已经对克氏锥虫的锥鞭毛体与纯化的大鼠嗜酸性粒细胞、中性粒细胞和巨噬细胞之间的相互作用进行了研究。由葡聚糖诱导的巨噬细胞被感染，寄生虫以无鞭毛体的形式繁殖，最终以锥鞭毛体的形式释放到培养基中。嗜酸性粒细胞表现出抗体依赖性吞噬作用，而中性粒细胞的吞噬作用则因抗体而增强。电子显微镜显示，一旦进入粒细胞吞噬体，锥鞭毛体就会转化为无鞭毛体，作为退化的前奏。当明显来自颗粒的物质在吞噬体中积累时，就会发生这种情况。嗜酸性粒细胞有能力将未完全退化的无鞭毛体喷射到培养基中。结合先前关于嗜酸性粒细胞对鸡红细胞进行溶解的延时摄影研究以及嗜酸性粒细胞通过分泌囊泡释放颗粒内容物的报道，对这一现象进行了讨论。
