Bassemir U, Miltenburger H G, David P
Cell Tissue Res. 1983;228(3):587-95. doi: 10.1007/BF00211478.
Cultures of cell line IZD-MB 0503 from Mamestra brassicae were inoculated with nonoccluded nuclear polyhedrosis virus of Autographa californica (AcNOV). At 1 h postinoculation (p.i.) nucleocapsids were found in the cytoplasm near nuclear pores and within the nucleoplasm. Formation of virogenic stroma was observed at 7 h p.i. The first short empty capsids were seen at 10 h postinoculation (p.i.), followed by partially and completely filled nucleocapsids (11-12 h p.i.) with most capsids filled at 12 h and later. This suggests that nucleocapsid components, such as capsid and DNA-core, assemble in successive stages rather than simultaneously. Viral progeny, being released from the cells by budding, were observed at 13 h p.i.
用苜蓿银纹夜蛾无包涵体核型多角体病毒（AcNOV）接种甘蓝夜蛾的IZD-MB 0503细胞系培养物。接种后1小时（p.i.），在靠近核孔的细胞质和核质内发现核衣壳。接种后7小时观察到病毒发生基质的形成。接种后10小时（p.i.）可见第一批短的空衣壳，随后是部分和完全填充的核衣壳（接种后11 - 12小时），大多数衣壳在12小时及以后填充。这表明核衣壳成分，如衣壳和DNA核心，是分阶段组装而非同时组装。接种后13小时观察到通过出芽从细胞中释放出病毒后代。
