Nishimura T, Favre D, Dürrenberger M, Michel M R, Ichihara I, Koblet H
Department of Anatomy, Aichi Medical University, Japan.
Okajimas Folia Anat Jpn. 1994 Aug;71(2-3):83-97. doi: 10.2535/ofaj1936.71.2-3_83.
Translocation of baculovirus nucleocapsids (45 nm in diameter, approximate length of 280-300 nm) from nucleoplasm to cytoplasm was studied morphologically using cryofixation and gold labeled wheat germ agglutinin (WGA-gold) during recombinant Autographa californica nuclear polyhedrosis virus infection in Sf9 cells. Nucleocapsids formed in the nucleoplasm migrated into protrusions of the nuclear envelope, but not into nuclear pore complexes. We found cross-like membranous structures. Small pores seemed to be in the protruding nuclear double membranes. The middle piece of a nucleocapsid was located within the small pore whereas the upper part was in the cytoplasm. Other nucleocapsids were situated within pores without colocalization of WGA-gold in the nuclear envelope. These results suggest that baculovirus nucleocapsids use small pores in the nuclear-derived membranes or incomplete nuclear pores in the nuclear envelope to migrate from the nucleoplasm to the cytoplasm, but not complete nuclear pore complexes proper.
在重组苜蓿银纹夜蛾核型多角体病毒感染Sf9细胞的过程中，利用冷冻固定和金标麦胚凝集素（WGA-金），从形态学角度研究了杆状病毒核衣壳（直径45 nm，长度约280-300 nm）从核质到细胞质的转运。在核质中形成的核衣壳迁移到核膜的突起处，但未进入核孔复合体。我们发现了十字形膜结构。在突出的核双层膜中似乎存在小孔。核衣壳的中部位于小孔内，而上部位于细胞质中。其他核衣壳位于孔内，核膜中没有WGA-金的共定位。这些结果表明，杆状病毒核衣壳利用核衍生膜中的小孔或核膜中不完整的核孔从核质迁移到细胞质，而不是利用完整的核孔复合体。
