Chandrasekhar S, Norton E, Millis A J, Izzard C S
Cell Biol Int Rep. 1983 Jan;7(1):11-21. doi: 10.1016/0309-1651(83)90100-5.
Analysis of fibronectin synthesized by human fibroblasts, at different times during serial subcultivation, reveals functional differences. Fibronectin isolated from late passage cells is defective in promoting cell adhesion, cell spreading, and the formation of focal contacts. These changes are not the result of an inability of late passage cells to interact with fibronectin, since late passage cells become adhesive and form focal contacts in the presence of fibronectin isolated from early passage cells. Therefore, we conclude that late passage cellular fibronectin derived from late passage cells cannot support the cell substrate interactions.
对人成纤维细胞在连续传代培养不同时间合成的纤连蛋白进行分析，揭示了功能差异。从传代后期细胞中分离出的纤连蛋白在促进细胞黏附、细胞铺展和黏着斑形成方面存在缺陷。这些变化并非传代后期细胞无法与纤连蛋白相互作用的结果，因为传代后期细胞在存在从传代早期细胞中分离出的纤连蛋白时会变得具有黏附性并形成黏着斑。因此，我们得出结论，源自传代后期细胞的传代后期细胞纤连蛋白无法支持细胞与底物的相互作用。
