Vogel K G, Kelley R O, Stewart C
Mech Ageing Dev. 1981;16(4):295-302. doi: 10.1016/0047-6374(81)90013-0.
Indirect immunofluorescence was used to investigate the production of a fibrillar fibronectin matrix by human diploid fibroblasts (IMR-90) as cells progress through their in vitro lifespan. Early and mid-passage cultures displayed a prominent fibrillar reticulum over the cell layer which formed within 24 hours of seeding. Even sparsely seeded early-passage cells exhibited fibrils of fibronectin on external surfaces. In contrast, fibrillar fibronectin was reduced or absent on surfaces of late-passage cells. However, the larger, non-proliferating, late-passage cells were producing fibronectin, 1h determined by radioimmunoassay of the medium.
采用间接免疫荧光法研究人二倍体成纤维细胞（IMR-90）在体外生命周期进程中纤维状纤连蛋白基质的产生情况。传代早期和中期培养物在接种后24小时内在细胞层上显示出明显的纤维状网状结构。即使是接种稀疏的传代早期细胞在其外表面也呈现出纤连蛋白纤维。相比之下，传代后期细胞表面的纤维状纤连蛋白减少或缺失。然而，通过对培养基进行放射免疫测定确定，较大的、不再增殖的传代后期细胞仍在产生纤连蛋白。
