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蛋白质导入线粒体。外膜生物发生的体外研究。

Import of proteins into mitochondria. In vitro studies on the biogenesis of the outer membrane.

作者信息

Gasser S M, Schatz G

出版信息

J Biol Chem. 1983 Mar 25;258(6):3427-30.

PMID:6339485
Abstract

The yeast mitochondrial outer membrane contains a major 29-kilodalton protein which is encoded in the nucleus. When this polypeptide is synthesized in vitro and then incubated with isolated yeast mitochondria, it binds to the organelles and becomes resistant to externally added trypsin. Post-translational insertion of the 29-kilodalton protein in vitro is not accompanied by NH2-terminal processing, appears to require neither ATP nor a transmembrane electrochemical potential, and is also observed with purified outer membranes, but not with yeast microsomes. In vitro insertion of the 29-kilodalton polypeptide is thus in several respects different from that of polypeptides destined for the internal compartments of the mitochondrion.

摘要

酵母线粒体外膜含有一种主要的29千道尔顿蛋白，该蛋白由细胞核编码。当这种多肽在体外合成，然后与分离的酵母线粒体一起温育时，它会与细胞器结合，并对外部添加的胰蛋白酶产生抗性。29千道尔顿蛋白在体外的翻译后插入不伴随氨基末端加工，似乎既不需要ATP也不需要跨膜电化学势，并且在纯化的外膜中也能观察到，但在酵母微粒体中则观察不到。因此，29千道尔顿多肽的体外插入在几个方面不同于定位于线粒体内腔的多肽的插入。

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1
Import of proteins into mitochondria. In vitro studies on the biogenesis of the outer membrane.蛋白质导入线粒体。外膜生物发生的体外研究。
J Biol Chem. 1983 Mar 25;258(6):3427-30.
2
Disruption of the outer membrane restores protein import to trypsin-treated yeast mitochondria.外膜的破坏可恢复经胰蛋白酶处理的酵母线粒体的蛋白质导入。
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In vitro synthesis and integration into mitochondria of porin, a major protein of the outer mitochondrial membrane of Saccharomyces cerevisiae.酵母线粒体外膜主要蛋白孔蛋白的体外合成及导入线粒体过程
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Deletion of the receptor MOM19 strongly impairs import of cleavable preproteins into Saccharomyces cerevisiae mitochondria.受体MOM19的缺失严重损害了可裂解前体蛋白导入酿酒酵母线粒体的过程。
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Import of transcription factor MTF1 into the yeast mitochondria takes place through an unusual pathway.转录因子MTF1导入酵母线粒体是通过一条不同寻常的途径进行的。
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The dimensions of the protein import channels in the outer and inner mitochondrial membranes.线粒体外膜和内膜中蛋白质输入通道的尺寸。
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Mitochondrial dysfunction leads to nuclear genome instability via an iron-sulfur cluster defect.
线粒体功能障碍通过铁硫簇缺陷导致核基因组不稳定。
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Yeast mitochondrial outer membrane specifically binds cytoplasmically-synthesized precursors of mitochondrial proteins.酵母线粒体的外膜能够特异性地结合细胞质中合成的线粒体蛋白前体。
EMBO J. 1983;2(7):1113-8. doi: 10.1002/j.1460-2075.1983.tb01554.x.
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The outer membrane of yeast mitochondria: isolation of outside-out sealed vesicles.酵母线粒体的外膜：外翻封口袋的分离。
EMBO J. 1983;2(7):1105-11. doi: 10.1002/j.1460-2075.1983.tb01553.x.
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