Sanyal A, Getz G S
Deparment of Pathology, University of Chicago, Illinois 60637, USA.
J Biol Chem. 1995 May 19;270(20):11970-6. doi: 10.1074/jbc.270.20.11970.
We report studies of the import into mitochondria of MTF1, a nucleus-encoded factor that markedly stimulates the specific transcription of mitochondrial DNA. Unlike most of the mitochondrial precursor proteins studied so far, import of MTF1 does not appear to require a receptor on the outer membrane, membrane potential across the inner membrane, or ATP hydrolysis. Its import is not affected by low temperature. It lacks a cleavable presequence but translocates across the inner membrane through its amino terminus; its sorting is independent of hsp60. Our results indicate an unusual and distinct import pathway for MTF1 into the yeast mitochondria.
我们报告了对MTF1导入线粒体的研究，MTF1是一种核编码因子，可显著刺激线粒体DNA的特异性转录。与目前研究的大多数线粒体前体蛋白不同，MTF1的导入似乎不需要外膜上的受体、内膜两侧的膜电位或ATP水解。其导入不受低温影响。它缺乏可裂解的前导序列，但通过其氨基末端跨内膜转运；其分选独立于hsp60。我们的结果表明MTF1进入酵母线粒体的途径不同寻常且独特。
