Ocklind C, Forsum U, Obrink B
J Cell Biol. 1983 Apr;96(4):1168-71. doi: 10.1083/jcb.96.4.1168.
We recently identified a 105,000-dalton plasma membrane glycoprotein, denoted cell-CAM 105 (CAM, cell adhesion molecule), that is involved in intercellular adhesion of reaggregating rat hepatocytes (Ocklind, C., and B. Obrink, 1982, J. Biol. Chem., 257:6788-6795). In this communication we used a monospecific rabbit antiserum against cell-CAM 105 to localize the antigen by indirect immunofluorescence on isolated rat cells and on frozen rat tissue sections. This antiserum stained the surface of freshly isolated hepatocytes. In liver sections, however, the fluorescence seemed to be located exclusively along the bile canaliculi. In addition, cell-CAM 105 showed a very specific tissue distribution. Thus a specific fluorescence was seen only in the epithelia of the stomach, the small intestine, the large intestine, the glandular epithelium of the parotid gland, and the tubules of the kidney. No specific fluorescence was found in variety of other tissues, including cartilage, interstitial connective tissue, smooth muscle, skeletal muscle, heart muscle, eye, brain, skin, the epithelia of oesophagus, bladder, uterin mucosa, thyroid follicles, prostate gland, or collecting ducts of the kidney. In the simple epithelia of the intestine and the kidney tubules the fluorescence was confined to the apical, luminal portion. Thus, both in these epithelia and in liver, cell-CAM 105 was confined to the apical, luminal portion. Thus, both in these epithelia and in liver, cell-CAM 105 was located where the typical junctional complexes between cells are found. These findings taken together with the fact that cell-CAM 105 is involved in intercellular adhesion between hepatocytes suggest with the fac that cell-CAM 105 is involved in intercellular adhesion between hepatocytes suggest that cell-CAM 105 is a member of the junctional complexes of hepatocytes and some simple epithelia.
我们最近鉴定出一种105,000道尔顿的质膜糖蛋白，命名为细胞黏附分子105（CAM，细胞黏附分子），它参与重新聚集的大鼠肝细胞的细胞间黏附（奥克林德，C.，和B. 奥布林克，1982，《生物化学杂志》，257:6788 - 6795）。在本通讯中，我们使用针对细胞黏附分子105的单特异性兔抗血清，通过间接免疫荧光在分离的大鼠细胞和冷冻的大鼠组织切片上定位抗原。这种抗血清对新鲜分离的肝细胞表面进行了染色。然而，在肝脏切片中，荧光似乎仅沿着胆小管分布。此外，细胞黏附分子105显示出非常特异的组织分布。因此，仅在胃、小肠、大肠、腮腺腺上皮和肾小管上皮中可见特异性荧光。在包括软骨、间质结缔组织、平滑肌、骨骼肌、心肌、眼睛、大脑、皮肤、食管上皮、膀胱、子宫内膜、甲状腺滤泡、前列腺或肾集合管在内的多种其他组织中未发现特异性荧光。在小肠和肾小管的单层上皮中，荧光局限于顶端的管腔部分。因此，在这些上皮组织和肝脏中，细胞黏附分子105都局限于顶端的管腔部分。因此，在这些上皮组织和肝脏中，细胞黏附分子105都位于细胞间典型连接复合体所在的位置。这些发现连同细胞黏附分子105参与肝细胞间细胞黏附这一事实表明，细胞黏附分子105是肝细胞和一些单层上皮细胞连接复合体的成员。
