Taylor S I, Underhill L H, Hedo J A, Roth J, Rios M S, Blizzard R M
J Clin Endocrinol Metab. 1983 Apr;56(4):856-61. doi: 10.1210/jcem-56-4-856.
[125I]Insulin binding has been studied with cultured cells from an insulin-resistant patient with the Rabson-Mendenhall syndrome. Previously, this patient has been demonstrated to have decreased insulin binding to circulating blood cells. Similarly, [125I]insulin binding to cultured lymphocytes and cultured fibroblasts was markedly decreased. Whereas the decrease in [125I]insulin binding to cultured lymphocytes appeared to result from a decrease in receptor number in cultured lymphocytes, there appeared to be a reduction in the affinity of [125I]insulin binding to cultured fibroblasts. This discrepancy between the observations with the two cell types is a general phenomenon which has been described in patients with a variety of syndromes of extreme insulin resistance. It is possible that the interpretation of the studies with the fibroblasts is complicated by the confounding influence of receptors for insulin-like growth factors on the surface of those cells. If the number of insulin receptors is sufficiently reduced on fibroblasts from the patient with extreme insulin resistance, then the low level of residual [125I]insulin binding may involve primarily receptors for insulin-like growth factors, which bind insulin with relatively low affinity.
利用来自一名患有拉布森 - 门登霍尔综合征的胰岛素抵抗患者的培养细胞，对[125I]胰岛素结合进行了研究。此前已证实，该患者与循环血细胞的胰岛素结合减少。同样，[125I]胰岛素与培养的淋巴细胞和培养的成纤维细胞的结合也显著减少。虽然[125I]胰岛素与培养淋巴细胞的结合减少似乎是由于培养淋巴细胞中受体数量减少所致，但[125I]胰岛素与培养成纤维细胞的结合亲和力似乎有所降低。这两种细胞类型观察结果之间的差异是一种普遍现象，在患有各种极端胰岛素抵抗综合征患者中均有描述。成纤维细胞表面胰岛素样生长因子受体的混杂影响可能使对成纤维细胞研究的解释变得复杂。如果来自极端胰岛素抵抗患者的成纤维细胞上胰岛素受体数量充分减少，那么残余的低水平[125I]胰岛素结合可能主要涉及胰岛素样生长因子受体，该受体与胰岛素的结合亲和力相对较低。
