Rordorf C, Gambke C, Gordon J
J Immunol Methods. 1983 Apr 15;59(1):105-12. doi: 10.1016/0022-1759(83)90150-3.
A dot immunobinding assay procedure has been developed for autoantibodies, and has been applied to the sera of MRL lpr/lpr mice. The profiles thus obtained include assays for circulating immune complexes, and antibodies against single-stranded DNA, double-stranded DNA, ribosomes, soluble nuclear deoxyribonucleoprotein, and retroviral antigens. Part of the data was compared with ELISA results. The anti-DNA assays were specific, as some individual sera show exclusively anti-double-stranded DNA specificity. The finding of anti-ribosomal antibodies in these mice extended the analogy between the murine disease and human systemic lupus erythematosus. The specificity of the anti-retroviral antibodies was examined following electrophoretic separation of the antigens and blotting on nitrocellulose. Previously undescribed classes of anti-retroviral antibodies were found. Circulating anti-retroviral protein p30 was found in all sera having high anti-retroviral titers.
已开发出一种针对自身抗体的斑点免疫结合测定方法，并已应用于MRL lpr/lpr小鼠的血清。由此获得的检测结果包括循环免疫复合物以及针对单链DNA、双链DNA、核糖体、可溶性核脱氧核糖核蛋白和逆转录病毒抗原的抗体检测。部分数据与酶联免疫吸附测定（ELISA）结果进行了比较。抗DNA检测具有特异性，因为一些个体血清仅显示抗双链DNA特异性。在这些小鼠中发现抗核糖体抗体，进一步拓展了小鼠疾病与人类系统性红斑狼疮之间的相似性。在抗原经电泳分离并印迹到硝酸纤维素膜上后，对抗逆转录病毒抗体的特异性进行了检测。发现了以前未描述过的抗逆转录病毒抗体类别。在所有抗逆转录病毒滴度高的血清中都发现了循环抗逆转录病毒蛋白p30。
