Knauer V, Hegerl R, Hoppe W
J Mol Biol. 1983 Jan 25;163(3):409-30. doi: 10.1016/0022-2836(83)90066-9.
From the micrographs of a tilt series, several particles of negatively stained 30 S ribosomal subunits of Escherichia coli were three-dimensionally reconstructed. Three of them showing similar orientation with respect to the supporting foil were averaged after alignment by newly developed three-dimensional correlation methods. As a main result we found a stained channel-like structure inside the particle. We tentatively propose that this corresponds, at least partially, to positively stained segments of the 16 S RNA.
从倾斜系列的显微照片中，对大肠杆菌30S核糖体亚基的几个负染色颗粒进行了三维重建。通过新开发的三维相关方法对齐后，对其中三个相对于支撑箔显示相似取向的颗粒进行了平均。作为主要结果，我们在颗粒内部发现了一个染色的通道状结构。我们初步提出，这至少部分对应于16S RNA的正染色片段。
