Ogawa K, Matsunaga M, Hamada H, Oohashi H, Pak C H, Morimoto K, Hara A, Kawai C
Jpn Circ J. 1983 Mar;47(3):294-9. doi: 10.1253/jcj.47.294.
The molecular weight (MW) of active renin (AR) and inactive renin (IR) was investigated in individual plasma samples from 15 essential hypertensive and 5 normal subjects. The patients with essential hypertension were classified into 3 subgroups: low plasma renin activity (PRA), normal PRA and high PRA. The MW of AR and IR was estimated by Sephadex G-100 gel filtration. IR in the eluates was activated with trypsin. Plasma renin activity and renin activity in eluates were measured by radioimmunoassay of angiotensin I. At least 3 sizes of AR (MW: 48,000, 53,000 and 57,000) and 2 sizes of IR (MW: 53,000 and 57,000) were discovered, and the MW of AR was less than or equal to the MW of IR. The larger AR was predominant in the low PRA group and the smaller one in the high PRA group. This was also true for IR. This relation was also observed in the 2 types (high renin and low renin) of pooled plasma. In high renin pooled plasma the MW of AR and IR was about 48,000 and 54,000, respectively, while both were about 56,000 in low renin pooled plasma. It appears that the larger type of AR and IR remains in the circulation in the suppressed renin state, and that the smaller type of renins enters into the circulation when the secretion of renins is enhanced.
对15例原发性高血压患者和5例正常受试者的个体血浆样本中的活性肾素（AR）和无活性肾素（IR）的分子量（MW）进行了研究。原发性高血压患者被分为3个亚组：低血浆肾素活性（PRA）、正常PRA和高PRA。通过Sephadex G - 100凝胶过滤法估计AR和IR的MW。用胰蛋白酶激活洗脱液中的IR。通过血管紧张素I的放射免疫测定法测量血浆肾素活性和洗脱液中的肾素活性。发现至少有3种大小的AR（MW：48,000、53,000和57,000）和2种大小的IR（MW：53,000和57,000），且AR的MW小于或等于IR的MW。较大的AR在低PRA组中占主导，较小的AR在高PRA组中占主导。IR也是如此。在两种类型（高肾素和低肾素）的混合血浆中也观察到了这种关系。在高肾素混合血浆中，AR和IR的MW分别约为48,000和54,000，而在低肾素混合血浆中两者均约为56,000。似乎较大类型的AR和IR在肾素受抑制状态下保留在循环中，而较小类型的肾素在肾素分泌增强时进入循环。
