Ustinova S E, Uchitel' I A
Kardiologiia. 1985 Jan;25(1):44-7.
The total renin activity (TRP) and inactive renin levels (IR) in the blood plasma were examined with the help of cryo- and trypsin activation in 9 normal subjects, in 40 patients with essential hypertension and in 18 patients with primary aldosteronism (PA). The cryoactivation method was shown to detect 40-80% of IR determined by the method of trypsin activation. Both methods revealed analogous ratios of the two renin forms in its total production in the presence of essential hypertension (in different "renin" subgroups) and arterial hypertension secondary to PA and may be used as the methods of choice when examining essential hypertension patients with the normal and subnormal plasma renin activity. The method of trypsin activation is more preferable for a more accurate quantification of small amounts of IR found in essential hypertension patients with a high plasma renin activity. With a high level of the TRP (over 8 ng/ml/h) activation should be performed in diluted plasma.
借助冷冻激活和胰蛋白酶激活法，检测了9名正常受试者、40名原发性高血压患者和18名原发性醛固酮增多症（PA）患者血浆中的总肾素活性（TRP）和无活性肾素水平（IR）。结果显示，冷冻激活法检测出的IR占胰蛋白酶激活法所测IR的40%-80%。在原发性高血压（不同“肾素”亚组）以及PA继发的动脉高血压患者中，两种方法均显示两种肾素形式在其总生成量中的比例类似，并且在检测血浆肾素活性正常和低于正常的原发性高血压患者时，这两种方法均可作为首选方法。对于血浆肾素活性高的原发性高血压患者中少量IR的更准确定量，胰蛋白酶激活法更为可取。当TRP水平较高（超过8 ng/ml/h）时，应在稀释血浆中进行激活。
