Tomino Y, Endoh M, Miura M, Nomoto Y, Sakai H
Acta Pathol Jpn. 1983 Jan;33(1):113-22. doi: 10.1111/j.1440-1827.1983.tb02104.x.
A study on the immunopathological similarities between IgA nephropathy and Henoch-Schoenlein purpura (HSP) nephritis is described. Various examinations were performed as follows. (1) Pathological studies: light microscopic findings and immunofluorescent staining; (2) Measurement of the levels of IgA in pharyngeal washings and sera, and those of IgA quantitated by radial immunodiffusion; (3) Elution studies: renal biopsy specimens obtained from patients with IgA nephropathy and HSP nephritis were treated with citrate buffer (pH 3.2) and the "eluate" was neutralized by sodium hydroxide. The "eluate" was then applied to the acid-treated sections obtained from the same and other patients with IgA nephropathy as well as sections from patients with HSP nephritis and other glomerular diseases. The sections were stained with FITC-conjugated heavy chain specific antihuman IgA antisera and then examined with a fluorescent microscope. There were no differences in pathological findings of IgA nephropathy and HSP nephritis in the light microscopic and immunofluorescent examinations. The levels of IgA in pharyngeal washings and sera were significantly increased in patients with both diseases. IgA antibodies deposited in kidneys from patients with HSP nephritis crossreacted with kidneys from some patients with IgA nephropathy, and vice versa. However, antibodies from patients with IgA nephropathy and HSP nephritis did not react with normal glomeruli or other nephritic glomeruli. It is concluded that there are some immunopathological similarities between IgA nephropathy and HSP nephritis.
本文描述了一项关于IgA肾病与过敏性紫癜（HSP）肾炎免疫病理学相似性的研究。进行了如下各项检查：（1）病理研究：光学显微镜检查结果及免疫荧光染色；（2）测定咽洗液和血清中IgA水平，以及通过放射免疫扩散法定量的IgA水平；（3）洗脱研究：从IgA肾病患者和HSP肾炎患者获取的肾活检标本用柠檬酸盐缓冲液（pH 3.2）处理，“洗脱液”用氢氧化钠中和。然后将“洗脱液”应用于取自相同及其他IgA肾病患者的经酸处理的切片，以及HSP肾炎患者和其他肾小球疾病患者的切片。切片用异硫氰酸荧光素（FITC）偶联的重链特异性抗人IgA抗血清染色，然后用荧光显微镜检查。在光学显微镜和免疫荧光检查中，IgA肾病和HSP肾炎的病理结果没有差异。两种疾病患者的咽洗液和血清中IgA水平均显著升高。HSP肾炎患者肾脏中沉积的IgA抗体与一些IgA肾病患者的肾脏发生交叉反应，反之亦然。然而，IgA肾病和HSP肾炎患者的抗体与正常肾小球或其他肾炎性肾小球不发生反应。得出的结论是，IgA肾病和HSP肾炎之间存在一些免疫病理学相似性。
