DeMoll E, Shive W
Biochem Biophys Res Commun. 1983 Jan 14;110(1):243-9. doi: 10.1016/0006-291x(83)91286-x.
The comparative ability of Escherichia coli K-12 hpb lambda-, a biotin overproducing strain, to incorporate 35S from isotopically labeled L-methionine, L-cystine, and the sulfane sulfur of thiocystine was determined. Comparison of the specific activity of sulfur in biotin produced by the organism with that of the 35S-labeled amino acids demonstrates that the sulfur of cystine is transferred to biotin with an efficiency of at least 75%, that the sulfur of methionine does not contribute to biotin significantly, and that the sulfane sulfur of thiocystine contributes approximately one-third of the sulfur to newly synthesized biotin and is essentially equivalent in utilization to that of the other two sulfur atoms in the molecule.
测定了生物素高产菌株大肠杆菌K-12 hpb λ-从同位素标记的L-甲硫氨酸、L-胱氨酸和硫代胱氨酸的硫烷硫中掺入35S的相对能力。将该生物体产生的生物素中硫的比活性与35S标记氨基酸的比活性进行比较表明，胱氨酸的硫以至少75%的效率转移到生物素中，甲硫氨酸的硫对生物素的贡献不显著，硫代胱氨酸的硫烷硫对新合成的生物素贡献约三分之一的硫，并且在利用上与分子中的其他两个硫原子基本相当。
