Dobitchin P D, Kirillov S V, Noskin V A, Peshin N N
Biophys Chem. 1983 Mar;17(2):165-9. doi: 10.1016/0301-4622(83)80010-6.
Spectral analysis of light scattered from solutions of 30 S subunits was performed by the method of regularization of the inverse spectral problem. The subunits observed under ionic conditions which preserved their biological activity (200 mM NH4Cl at 1 mM MgCl2) revealed a monodisperse pattern of scattering with diffusion constant D = (1.83 +/- 0.10) X 10(-7) cm2/s. The polydispersity and compaction of 30 S subunits were observed under inactivation ionic conditions (30 mM NH4Cl at 1 mM MgCl2). The number of compacted particles correlates with the irreversible loss of biological activity, the ability of 30 S subunits to bind specific tRNA.
采用逆谱问题正则化方法对从30 S亚基溶液散射的光进行光谱分析。在保持其生物活性的离子条件下（200 mM氯化铵，1 mM氯化镁）观察到的亚基显示出具有扩散常数D = (1.83 +/- 0.10)×10(-7) cm2/s的单分散散射模式。在失活离子条件下（30 mM氯化铵，1 mM氯化镁）观察到30 S亚基的多分散性和紧密性。紧密颗粒的数量与生物活性的不可逆丧失、30 S亚基结合特定tRNA的能力相关。
